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KMID : 0350519940470041793
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1793 ~ p.1805
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Detection of Dimethyl Sulfoxide from Perilymph and Morphological Study of Cochlear Hair Cells after its Instillation into External Auditory Canal in Guinea Pigs
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Abstract
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Dimethyl sulfoxide is a potent membrane active solvent, There have been several reports on the effect of dimethyl sulfoxide mixing with other drugs to skin, kidney and central nervous system, but reports abot its effect on the ear is scarce.
This study was planned to observe whether dimethyl sulfoxide could be detected from perilymph after instillation into external auditory canal or not, and to evaluate the amount of detected dimethyl sulfoxide from perilymph in comparison with from
serum.
Also the pathologic study was done by transmission electron microscope.
Healthy, seventy six guinea pigs, weighing between 250g and 350g each, were used in this study. They were divided into four groups-normal control group, intravenously injected group, external auditory canal-instilled group for detection of given
drug ,
group for pathologic study, and each group was subdivided according to using method and observing time.
Dimethyl sulfoxide(100%, 0.3ml) was given intravenously and was instilled into external auditory canal. Dimethyl sulfoxide was detected by gas chromatography from both serum & perilymph in 3 former groups on 20 minutes, 1 hours, 4 hours and 8
hours
after medication.
We also observed pathologic change of cochlear hair cells by transmission electron microscope ingroup for pathologic study on 2 weeks after medication.
@ES The results were as follows:
@EN 1. Dimethyl sulfoxide instilled into external auditory canal was detected from perilymph.
2. The amount of detected dimethyl sulfoxide from perilymph was less than that from serum in intravenously injected group and was more than that from serum in external auditory canal-instilled group.
3. Maximal detection time of dimethyl sulfoxide was 1 hours after injection in intravenously injected group and 4 hours after instillation in external auditory canal-instilled group.
4. There was no pathologic change of cochlear hair cells in both intravenously injected group and external auditory canal-instilled group.
Therefore in can be a good method of chemical labyrinthectomy for Meniere's disease to instill dimethyl sulfoxide mixing with aminoglycoside antibiotics into an external auditory canal.
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